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GPT neurons (lacZ) in ablated mice were also first detected at E12, but their density did not increase notably until E14 with the subsidence of early-onset apoptosis in unscarred dorsal pallium.

All mice were first used at 8 weeks of age and were maintained under SPF conditions at the Common Research Facility of the University of Glasgow.

This pathway specific regulation is even more pronounced when IL-6 is the stimulus (see below).Although differences between MRL and lpr mice were first seen at 10 weeks of age, the disparity in signaling responses became even greater at 15 20 weeks.

At necropsy, all mice were first weighed to obtain an overall measure of body size and then dissected and their organs weighed to the nearest 0.01 gram using digital scales.

The results for the three groups of mice were similar at the first two intervals.

The mice were sacrificed at the first appearance of morbidity, loss of weight (>10%), neurological abnormalities, failure to thrive or diarrhea.

For isolation of tumor cells, mice were sacrificed at the first therapy endpoint (see Figure  1 and Additional file 1: Table S1) with CO2.

Congenic mice were used at the second generation after being made homozygous.

For histological assessment, mice were sacrificed at day 42 post first CII immunization, and both hind limbs were removed.

To assess the persistence of mucosal IgA responses using this vaccine in a four week protocol, mice were tested at 4 weeks after the first dose then tested a second time, 14 weeks after the initial dose.

Dox was continued in the first group and withdrawn in the second, and mice were euthanized at d80.