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Experimental mice were analyzed after fourteen days of cohabitation.

Circadian periods of nose poking activity in mice were analyzed for each animal as described in Maggi et al.23.23

(d i) The level of pro-inflammatory cytokines from lung tissues of mice were analyzed by ELISA.

(B) Brain samples of WT and GFP+ mice were analyzed with GFP/FSC plot to determine the threshold of GFP signal for further flow cytometry analysis.

Thymocytes and splenic T cells from C57BL/6 mice were analyzed for their content of glycosphingolipids (GSL) by high performance liquid chromatography.

To test for possible immunogenicity, the blood stream cytokine profiles of mice were analyzed at 24 hours and two weeks after the CRISPR-Gold injection.

Fourteen homozygous LAMB3 628G>A knockin mice were analyzed.

(A B) HSCs and HPCs from 2 3 months old mice were analyzed by FACS.

More than 200 fibers from RNF13-/ and RNF13+/+ mice were analyzed.

The mice were analyzed under well-fed conditions or after 24 h of fasting.

At least 6 pairs of mice were analyzed for each assay.