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Therefore, the mice were allocated to one of the three groups: mice without adenovirus injection + ND, mice injected with adenovirus containing GFP (Ad-GFP) + HFD (high-fat diet, 60% kcal from fat, TD.06414) (Harlan Teklad, IN, USA), and mice injected with adenovirus containing JMJD2B (Ad-JMJD2B) + HFD.
Two days after surgical procedure mice were allocated to two treatment groups as per above and were monitored for tumor recurrence.
Based on whole body adiposity measurements by MRS and body weight data, mice were allocated into groups of four and housed in individually ventilated cages.
Two days after tumor resection, mice were allocated to 1 of 2 groups as previously described and observed for tumor recurrence (Figure 8D).
Ten days after injection (a time-point by which 95 100% of mice develop established lung metastases) mice were allocated to two treatment groups as per above.
When tumour growth had reached the desired size, mice were allocated into treatment groups ensuring that there was no statistical difference between each group's mean body weight and tumour volume.
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Twelve-week-old NZB/W F1 (NZ) and C57BL/6 J mice (controls) were allocated to receive HCQ or vehicle for 6, 12, or 18 weeks.
Balb/c mice or SD rats were allocated to three groups (n = 10) and subcutaneously (s.c).
Experiment 2. Fourteen 12-week-old apoE−/− male mice (weight 25 gr) were allocated into two groups with seven animals per group; the control group was fed chow diet and the VAD group was fed a vitamin A-deficient diet.
Experiment 1. Sixty-eight 12-week-old apoE−/− male mice (weight 25 gr) were allocated into 4 groups, 17 animals per group, according to their body weight, plasma cholesterol, and triglyceride (TG) concentrations.
In the first experiment, young mice (12 weeks old) were allocated into 3 groups: (1) low-fat diet; (2) low-fat diet + Dunaliella powder (8%); (3) low-fat diet + β -carotene-deficient Dunaliella.
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