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From treatment day 4 8 mice were additionally injected with DTIC or saline.
The six mice were imaged at 4, 24, 48, 72 and 96 h post-injection (pi), and two mice were additionally imaged at 168 and 197 h post-injection.
Therefore, animals of three strains of mice were additionally characterised regarding CMS effects on anxiety-related behaviour.
In the case of mice arising from the R6/2×Hdac7+/− cross, all cages contained at least one mouse from each genotype and mice were additionally given mash consisting of powdered chow mixed with water from 12 weeks of age.
Livers of those mice were additionally examined by a pathologist for GVHD symptoms.
For histology, mice were additionally perfused with 4 % PFA in PBS.
Similar(44)
Intriguingly, the cystic kidney phenotype observed in Fat4 mutants is accentuated when mice are additionally halpoinsufficient for Vangl2 (45).
While SCID mice lack mature B- and T-lymphocytes due to a recombinase gene deficiency (Bosma et al, 1983) NOD/SCID mice are additionally characterised by a deficit in NK-cells and functioning macrophages (Shultz et al, 1995).
When comparing the GluN2B KI mice with CaMKII T286A or CaMKII knock-out mice in other behavioral tasks in future studies, it may have to be taken into account that the GluN2B KI mice are impaired only in LTP, while the CaMKII mutant mice are additionally impaired in LTD.
Each mouse was additionally inoculated with one negative control (10 CFU of non-fluorescent bacteria) to differentiate the signal from autofluorescence or background signal.
Some mice with elevated IOPs were additionally age determined prior to assessment of the optic nerve.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com