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Prior to ERG testing, mice were adapted to dark overnight.
After a 3-week interval, we performed a second and third set of experiments in which CXCL14−/− mice were adapted to this experimental procedure such that they returned to normal feeding.
For whole body O2 consumption assessment, mice were adapted for 8 hours in the metabolic chamber before reading were taken periodically at 15 min intervals for the next 10 h.
Prior to testing, the mice were adapted to the room with lights on for 15 min. The main objective of performing these sensorimotor tasks was to verify that any treatment related effects observed in the cognitive tasks could not be explained by differences in sensorimotor abilities.
Induced pluripotent stem cells (iPSCs) generated from adult fibroblasts of X-CGD mice were adapted to feeder-free condition for five passages and subsequently characterized for stem cell morphology (round shape, large nucleus, and scant cytoplasm), alkaline phosphatase activity, and expression of pluripotency markers Sox2, Oct4, Klf4, Nanog, SSEA-1, and c-Myc (Fig. 1A).
Prior to each day's testing, the mice were adapted to the room with lights on for 15 min. For each session, all arms were baited with saccharine flavored water, and animals were permitted to enter all arms until the eight rewards had been consumed.
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A low-calorie diet could be much closer to the diet that mice are adapted to in the wild, and therefore it could extend life simply because it is much healthier for them.
The protocol for imaging infected SMG in living mice was adapted from Purves and Lichtman [15], [40].
Meal patterns for Sox2- Cre: Errβ lox / lox mice are adapted from [ 14].
The other group of mice was adapted to 18°C for 1 week prior to exposure to 4°C.
Mouse models were adapted with wild-type EBOV by serial passage [ 41].
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