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During suckling, JunB-KO mice gained significantly less weight than their WT littermates, and at the age of 3 months, JunB-KO mice weighed approximately 25% less than their WT littermates (Fig. 1A).
This dosing was used based on pilot work in 3- to 4-month-old mice (Fig. S3A) and because the 23-month-old mice weighed approximately twice as much as the 5-month-old mice at the start of the experiment.
Mice weighed approximately 25 g at the beginning of the therapy.
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Groups of five mice, weighing approximately 25 g each, were injected with 200 μl of the above suspension in the left thigh muscle.
Results indicate that the obese mice weigh approximately twice as much as the lean mice, but the quadriceps muscles of the ob/ob mice are 40% smaller than those of controls.
Female BALB/c mice (weighing approximately 20 g) were housed under standard conditions of light and temperature and were fed standard laboratory chow and water ad libitum.
Two wild-type adult male C57BL/6 mice weighing approximately 27 g were chosen for imaging.
Male C57BL6 mice weighing approximately 20-30 grams were purchased from Charles River (Kingston, NY).
Male C57BL/6JBomTac mice, weighing approximately 25 g, were obtained from Taconic Europe (Ejby, Denmark).
Non-invasive in vivo images were acquired from pinna of healthy ~8 weeks old male hairless mice weighing approximately 28g.
Twenty Swiss male albino mice weighing approximately (20 ± 1.0 g) were taken and these mice were divided into four groups of five mice each.
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