In addition, as patterned auditory stimulus without ethological relevance to mice we used piano music by Mozart (KV 448).
Regarding the effects of GO on mice, we used tail vein injection pathway to evaluate the in vivo toxicity.
Regarding the effects of FMNPs on lifespan of mice, we used tail vein injection pathway to evaluate the in vivo toxicity of FMNPs.
To identify conserved intronic sequences expressed in both humans and mice, we used custom-designed human cDNA microarrays to separately interrogate RNA from mouse and human liver, kidney, and prostate tissues.
To evaluate this in 3xTg-AD mice, we used the novel-object recognition task.
For infections in mice we used the same protocol described by Andrade [2].
As reporter mice, we used mice that were heterozygous for the targeted mutation.
Thus, the TH-MYCN transgenic mice we used in this study are the same lineage commonly used in neuroblastoma studies.
While Spriggs' group used a transgenic knockout mice, we used a passive transfer strategy in a severely immunocompromised mice (Rag1−/−).
To identify the isoform responsible for the augmented fluorescent response in mutant mice we used specific NOS inhibitors.
For the genetic analysis of VRK1 in mice, we used VRK1-deficient mice as previously reported (Figure S1) [17].
