To determine the levels of administered tumor suppressor miRNAs in mouse intestine, we exploited the fact that the synthesized miRNAs used in our experiment, like plant-produced miRNAs, are methylated at the 2′ position of the ribose of the 3′ terminal nucleotide, whereas endogenous mouse miRNAs are not.
To investigate the functional differences between the two isoforms of the ZFY genes in mice and humans, we exploited the fact that the acidic domains of Zfy2 and Zfx have been shown to activate transcription in the yeast Saccharomyces cerevisiae when fused to the DNA-binding domain of the Gal4 transcriptional activator (5).
In earlier work in LSD mice and dog models, we exploited the utility of adeno-associated viruses (AAVs) to transduce brain ventricular lining cells (ependyma) for secretion of lysosomal hydrolases into the cerebrospinal fluid (CSF), with subsequent distribution of enzyme throughout the brain resulting in improved cognition and extending lifespan.
To tease apart the main features associated with the inhibition of tumor progression we exploited mice challenged with TUBO cells.
To elucidate the potential role of VEGFR2 activation in angiogenesis in a mouse model of ICH, we exploited the VEGFR2 inhibitor, SU5416 in our studies.
In this study, we exploited transgenic mice expressing a FRET biosensor of ERK and monitored ERK activity in epidermal cells at single cell resolution.
Given the crucial role of DC in priming Plasmodium-specific CD8+ T cells in general and in the dermis in particular (Chakravarty et al, 2007), we exploited transgenic mice, whose CD11chi DC subsets can be depleted conditionally, to demonstrate that these cells play an essential role in the sterile immunity conferred by s.c. immunization.
In order to exploit the recent development of transgenic mice, we have adapted to the mouse the rat aortic ring assay in collagen gel.
We exploited the mouse model with conditional ablation of Fig4 in Schwann cells to investigate whether the resulting impairment of endolysosomal trafficking would influence myelin biogenesis during development.
To address the role of Raidd in tumorigenesis in more detail, we exploited different mouse models where we induced thymic lymphomas by γ-irradiation, fibrosarcomas by 3-methylcholanthrene (3-MC) injection or B-cell lymphomas by aberrant expression of the c-Myc proto-oncogene.
