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On average, there was no significant accumulation of QD outside of the bladder, although in some mice we detected extravesical biodistribution of QD suggesting a route for systemic exposure under some conditions.
Although we find differentiation of proximal epithelium is also affected in lungs of Creb1−/− mice, we detected only sparse activation of Creb1 in these cells.
In the lungs of these mice, we detected myeloid (CD11b+) cells in numbers comparable to those of lung infiltrating BMDCs in BMT-Actb-GFP/C57BL mice.
In the islets of adult p21-overexpressing transgenic mice, we detected the expression of CD133 and c-Met two days after STZ injection.
In fact, in the thymus and spleen of the 5CC7 RAG-2-deficient donor mice, we detected rare T cells expressing non-Tg TCR chains.
When comparing the protein patterns of APP23 with wildtype mice, we detected a relatively large number of altered protein spots at all age stages and brain regions examined which largely preceded the occurrence of amyloid plaques.
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Second, in mouse we detected Nfatc2 transcripts lacking exon II (ΔII).
However, using a fluorescent reporter mouse, we detected Cre activity mainly in epithelial cells throughout the whole mouse body, but there was little or no activity in hematopoietic or mesenchymal cells.
Although we have not yet examined the methylation status of other CpG sites, such as those at positions -298, -263, -60, +89 and +158, the level of methylation at these sites may be similar or increased in the db/db and DIO mice relative to the levels in WT mice, since we detected reduced PPARγ mRNA expression in these diabetic mouse models.
We performed quantitative analysis of early pachytene cell fractions in control and treated mice, and we detected three major defects in ATZ-treated mice: (1) incomplete synapsing of one or two chromosomes, which was often detected together with the formation of branched chromosome structures; (2) sex-body defects (sex chromosomes with very long chromosomal axes (Additional file 1: Figure S4A).
By day 15th or 30th, in all mice groups we detected a high frequency of IFN-γ producing specific T cells (Fig. 3F).
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