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Using the ΔΔCt values for samples from control AA mice, we calculated the relative quantities of HO-1 and NQO1 mRNA for the various organs from AS and SS mice.
In the experiments utilizing TH-EGFP mice, we calculated the relative brightness of TH ICC in biocytin-labeled neurons by analyzing fluorescent signal intensity using the image analysis software ImageJ (NIH).
To examine the pattern of LD observed in CD-1 mice, we calculated the square correlation coefficient (r2) between pairs of SNPs genotyped in Cohorts 1 and 2 independently using Haploview [36].
To quantify the gap detection ability of control and noise-exposed mice, we calculated the gap startle ratio before and after exposure.
For translational purposes and confirmation of the internal consistency of our behavioural readouts in aged mice, we calculated intercorrelations between the observed behavioural sub-phenotypes catatonia, depression, loss of interest, impaired social interaction and anxiety as target variables.
As a measure of scatter among individual mice, we calculated the coefficient of variation (CV), i.e. the standard deviation of the mean from qPCR measurements of individual organs in percent.
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To assess learning, for each mouse we calculated the distance between the mouse and the platform every 0.5 sec until the mouse reached the platform.
For each miRNA for each mouse, we calculated the delta Ct relative to miR-17*.
In order to better understand the evolutionary relationships between the feline cDNA sequences and the orthologous sequences in dog, human and mouse, we calculated dN/dS values for orthologous sequences across the different species.
To determine the possible positions of the virtual mouse that reflected the relative positions of vocally interacting mice in the multiple mouse experiment, we calculated the distance between each vocally interacting male and female at the time of the female vocalization as well as the orientation of the female relative to the male.
While the in vitro assay reported no synergy between the two drugs, the in vivo mouse study showed some degree of synergy: reanalyzing the mouse data, we calculated an S value of 30 and 3.22 respectively, using either the mortality or maximum weight loss as a metric.
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CEO of Professional Science Editing for Scientists @ prosciediting.com