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This effect was transient, as food intake of CXCL14-injected CXCL14−/− mice was returned to normal levels on the second day.
One group of mice was returned to context A for 3 min and the other group of mice was placed in context B for 3 min. Context B was modified from context A by altering the shape of the chamber with a curved plastic board, changing the olfactory cues, and changing distal visual cues with posters on the walls of the sound attenuating box.
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Mice were allowed to explore the objects for 5 min, after which the mice were returned to their own cages for a 5-min retention period.
Mice were allowed to explore the objects for 10 min, after which the mice were returned to their home cage.
After 2 min, mice were returned to the arena, and left to explore for 15 min. Mice were then removed from the arena and returned to the home cage.
The control mice were returned to their home cages immediately after pairing training and left undisturbed.
This effect disappeared following repeated exposure to the same environment, but then reappeared if mice are returned to the same environment 10 14 days later.
Echocardiography was performed under a brief period of isoflurane anaesthesia before and immediately after re-warming, and then the mice were returned promptly to their metabolic cages where oxygen consumption recordings were resumed within 1 to 2 min.
40 mice were returned to the island in late 2006 with the remaining being re-introduced in stages.
Thereafter, mice were returned to their new testing cage.
One day after conditioning, mice were returned to the chamber.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com