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Food intake of diet-restricted ob/ob and DKO mice was restricted to 2.5 g/day, which is the normal daily intake of lean WT mice, between 12 and 24 weeks of age [ 11].ACE-inhibition was obtained with captopril (10 mg/kg/day) intraperitoneally from 12 until 24 weeks of age in WT, ob/ob and DKO [ 22].
The increased susceptibility of immunized mice was restricted to peripheral prion exposure, and was not observed after i.c.
To minimize stress, head-fixation of habituated mice was restricted to <3 hr.
First of all, the immunological response in BALB/c mice was restricted to the oxazolone-treated group.
The analysis demonstrated that most cleaved caspase-8 labeling in LPS-injected Casp8 fl/fl mice was restricted to CD16/32-labeled CD16/32-labeled 3a, b).
This is in agreement with the presented data, because MMP9 expression in the lesions found in Plg −/− mice was restricted to granulocytes.
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These results suggest that insulin signaling defects in dmpk−/− mice are restricted to DMPK-expressing tissues.
The mice were restricted from food and water four hours prior to each inoculation to reduce variation in systemic invasion due to food in the gut.
As reported in Fig. 4, all groups of mice generated a comparable TRP-2-specific CTL response, therefore confirming that tolerance in TRAMP and TRAMPJα18−/− mice is restricted to the PC-associated antigen.
As increased physical activity in KRAP−/− mice is restricted to dark period (Figure 2B), the higher physical activity does not fully account for the increased whole-body energy expenditure, suggesting that other processes such as heat production will be involved in the altered energy homeostasis in KRAP−/− mice.
Although there were relatively long-term differences in absolute body weights, an analysis of body weight changes over the P2 P14 period showed that significant losses of body weight in the PCP-treated mice were restricted to the first post-treatment day (data not shown).
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