For the tail flick test, mice were restrained in a tube with only it's hind legs and tail protruding.
To apply the stimulus, mice were restrained by hand in a supine position and slowly stroked with the stimulus until it bubbled at the nostrils, stroked again, and replaced in the cage.
Conscious mice were restrained, and the sensor was placed at the base of the tail.
The mice were restrained from food and water intake for 1 hr after inoculation.
In the holders, mice were restrained, but not anaesthetized during irradiation.
For lung irradiation, anesthetized mice were restrained in modified Perspex tubes.
In order to make the hole the mice were restrained for 20 s.
During light treatment, mice were restrained unanaesthetised in special holders designed to expose the sacral region of their backs.
The anesthetized mice were restrained in a supine position on a heated pad to maintain the body temperature at 37°C.
Briefly, mice were restrained, the tail immersed in a water bath, and the latency to respond recorded.
