Thereafter, mice were regularly checked on pain stimuli and discomfort.
Tumor sizes and body weights were recorded twice weekly, and the mice were regularly observed over the course of the study.
Additionally, sentinel mice were regularly tested by Yale Rodent Services and found to be negative for common murine pathogens in the serum, nasopharynx and the gastrointestinal tract (pathogens tested included mouse hepatitis virus, parvovirus, mycoplasma pneumonia, sendai virus, lymphocytic choriomengitis virus, ectromelia virus, pneumonia virus and epizootic diarrhea of infant mice).
During the intervention, the mice were regularly monitored for weight, food consumption, and physical endurance.
The incision was closed using Vetbond (3M) and mice were regularly monitored throughout the course of infection.
DBA/1 mice were regularly weighed from the day of priming and checked for the development of arthritis after booster immunization.
The mice were regularly examined, tumor sizes were measured with a caliper, and tumor volumes were determined using the following formula: volume = 0.5 × (width)2 × length [ 26].
The mice were regularly screened for common pathogens and housed in compliance with guidelines set by the Experimental Animal Board under the Ministry of Agriculture of Norway.
Though only very few combined therapy treatment protocols have been tested so far, clear increases in survival time of MDR-tumour-bearing mice were regularly obtained, leaving hope for major improvement of the therapy using other dosing schedules.
However, male house mice are regularly exposed to a risk of sperm competition within their polygynous mating system because females often seek extra-territorial copulations that can result in multiply sired litters (Dean et al. 2006).
