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Liver of mice was quickly removed, cleaned, weighed and used for biochemical and histological studies.
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But Shih's team discovered that DNA nanodevices injected into the bloodstream of mice are quickly digested.
Sham-or RF-exposed mice were quickly sacrificed and the cerebral cortex was rapidly dissected from each brain.
In brief, brains of 21 days old mice were quickly removed and the neocortex was homogenized in 1 ml buffer containing 25 mM Tris-HCL (pH 7.4), 1mM EDTA and 1mM EGTA.
Hearts from WT and MED13cTg mice were quickly excised and arrested in ice-cold saline.
Nkx2.1-Cre::MADM mice were quickly decapitated and 300 μm coronal slices were prepared using a Leica VT1200-S vibratome.
Brains from ∼6 8 week old C57BL/6x129 mice were quickly removed and submerged in ice-cold oxygenated cutting solution.
Then mice were quickly translocated into the imaging chamber of the Maestro in vivo fluorescence imaging system (CRI, Woburn, MA, USA).
Briefly, cerebella from post natal days 6 (P6) WT, P2Y1R-null and GD3 synthase-null mice were quickly removed from skulls and placed in ice-cold DPBS (Dulbecco's PBS, pH 7.4; Cellgro).
Following deep anesthesia, mice were quickly decapitated, either immersion-fixed (P0 P4 animals) or perfusion-fixed (P6 P14) through the heart using 4%% phosphate-buffered paraformaldehyde (0.1 M PB, pH 7.4).
The liver, kidneys, heart, lung, spleen, right gastrocnemius muscle, and adipose tissue (epididymal) of each mouse were quickly excised and weighed.
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