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Total RNA (10 ug) from 46 embryonic kidney (15.5 dpc) from 5 litters of CD1 mice was put through one round of poly (A) selection (Oligotex Kit, Qiagen) followed by ribosomal depletion (Ribominus Kit, Invitrogen) to select mRNA.
For VLDL isolation, 400 μl plasma from fasted mice was put into the bottom of a 1-ml thick-walled polycarbonate tube, overlaid with 600 μl of 1.006 g/ml KBr solution, and centrifuged at 100,000 rpm at 16°C for 2 hr in a TLA 120.2 rotor.
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The mice are put to death with carbon dioxide, then frozen and shipped.
When the two sets of mice were put together in the same cage, the obese mice became lean.
If young mice are put in a maze several days in a row, most will learn where the escape route is and go right to it, said Dr. Eric L. Kandel, a professor at Columbia.
After the injection, the mice were put in a dark chamber for 4 h, and then tumor region were stripped from the mice.
In the third set of experiments, the mice were put down at 6 and 24 h for the control, "5 %," "20%%," and "100%%," ligated groups.
But differences did crop up once the grownup mice were put through a series of standard behavioral tests.
When the mice were put through the tests, they acted just as anxious as normal animals did.
The mice were put in a chamber where they experienced foot shocks, causing them to freeze in fear.
As related on the Israeli news site Arutz Sheva, the mice were put to the test during a December 2009 controlled experiment in which explosive charges were planted on twenty-two people scattered throughout a Tel Aviv mall.
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CEO of Professional Science Editing for Scientists @ prosciediting.com