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In separate experiments, the brain of injected mice was processed for biochemistry.
One in every five coronal sections covering either the frontal cortex, hippocampus, or amygdala of vector-injected mice was processed.
Broncheoalveolar lavage (BAL) fluid from influenza virus-infected mice was processed using a Luminex-based multiplex immunoassay kit (Procarta cytokine assay kit; Affymetrix, Santa Clara, CA, USA).
To obtain epithelial cells of high purity, the dorsal skin of male BALB/C mice was processed as previously described with slight modifications [ 33].
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End of life (EOL) mice were processed for histochemical analyses.
Six of the nine mice were processed for histopathological examinations.
Three mice were processed in each experimental group.
6 weeks post-operations, femurs from DAP12-/-FcRγ-/ mice were processed for histological examination.
Periovarial WAT from HFD-fed wildtype and HSL null mice were processed in parallel according to the following scheme.
Retinal sections of 12 months old mice were processed for immunohistochemistry using the streptavidin horse radish peroxidase complex.
Optic nerves from transcardially perfused mice were processed for light microscopy of semithin sections as recently reported [1].
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