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Exact(11)
50 µl of plasma from 5 mice was pooled for each set of case and control samples.
For each time point, RNA from 5 mice was pooled and hybridized in quadruplicates, using a dye swap design to produce 4 expression measurements per time point.
Lavage fluid from 2 or more mice was pooled and kept on ice until centrifugation for 20 min at 400×g at 4°C. Cell pellets were treated with sterile water for 10 seconds to lyse residual erythrocytes followed by a 1∶9 dilution of the water with M-SFM cell culture media.
Aortic valve tissue from three mice was pooled and RNA was isolated using the standard Trizol method.
RNA from the same organ types from three mice was pooled to compensate for potential individual variation.
The serum of mice was pooled per group and analyzed for the T-cell cytokines IL-2, IL-5, IL-6, IL-10, and IFN-γ.
Similar(49)
Urine samples collected from Ate1-deficient mice were pooled and compared with pooled urine from Ate1-containing mice.
Macrophages of normal mice were pooled and incubated with lemongrass essential oil (similar components to L. liversidgei chemotype II) at 37°C for 24 h.
Total RNA from 3 mice were pooled and one microarray was used for each pool.
Plasma from 5 mice with cancer and 5 control mice were pooled for further analysis.
Lymph nodes from two mice were pooled for TCR repertoire analyzes.
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