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The genome of the mice was modified either by optimization of the EDA natural splice sites or by deleting the EDA region.
Despite the lack of NPC1-YFP rescue in macrophages, cholesterol accumulation in the liver of R; N; Npc1 −/− mice was modified after a week of NPC1-YFP induction (Fig. 5E).
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These mice were modified to suppress EDG2, a protein whose absence causes schizophrenia.
A study in PTEN haploinsufficient mice [ 57] demonstrated that the phenotypes of these mice were modified in an additive fashion by SLC6A4 haploinsufficiency.
Whether muscle regeneration in aged mice is modified by changes in the extrinsic environment or by alterations of the satellite cells themselves is still a controversial issue.
In contrast, mice are modified by the genetic ablation of a gene of interest in an inducible manner to generate conditional knockouts with the use of the Cre/loxP phage recombinase system, for example [ 39].
The elastin preparation procedure for mouse organs was modified due to the small amount of material (0.02 0.10 g from Pcft-deficient mice [ 27] and 0.04 0.54 g from other mice).
An in situ hybridization protocol for mouse embryos was modified from [72] for use on EBs.
Thus in order to take full advantage of the affinity of the antibody for the podocyte, while at the same time preventing the antibody from inducing disease, the anti mouse podocyte was modified extensively as described below.
The mouse model development was modified as highlighted by Chang et al. [ 15, 40].
An ELISA assay for mouse anti-EGFP detection was modified from that of Brown et al (2001).
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