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The head of the mice was held against a V-shaped holder and the skin was pulled taut.
Of note, we found that the mass of sperm in the upper uterine horns of cKO mice was held within a dense, partially solidified mass that retained the tubular shape of the uterine horn following release into culture medium.
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The mice were held under typical laboratory conditions (temperature 22 °C ± 2 °C; 12 h light 12 h dark cycle and kept in plastic cages with free access to the commercial basal food and water.
The mice were held in a custom built restrainer during the measurements.
Briefly, the grip strength meter was positioned horizontally and mice were held by the tail and lowered toward the apparatus.
mTerc+/+ and mTerc−/− mice were held in a pathogen-free area (20 22°C) with free access to food and water.
The limb clasping test consists in analyzing the response of the mouse limbs when the mice are held suspended in the air by the tail.
Housing and experimental protocols were in accordance with the Swiss Animal Protection Law and mice were held in compliance with the regulations of the Veterinäramt, Kanton Zürich.
On their arrival in the laboratory, mice were held in standard laboratory cages in groups of 5 7 and randomly assigned to nine experimental groups (Fig. 1A and 5A).
Anesthetized mice were held in an inverted position for four minutes post-inoculation to allow virus to adsorb and to prevent immediate discharge of virus.
Similarly, all mice were held under a constant 12 h light-dark cycle, but the time schedules differed between laboratories (see Table 1).
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