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The fluorescence of FITC was not detected when mice was fixed with PFA fixative before the perfusion of FITC-containing saline.
Inguinal adipose tissue from 10 day old mice was fixed in formalin, embedded in paraffin; 10_µm sections were stained with eosin/hematoxylin and photographed at constant settings.
Freshly extracted pancreas from wild-type C57B/6 and ankyrin-B+/− C57B/6 mice was fixed for 18 hours at 4°C in 4% paraformaldehyde.
One series of frozen sections representing the entire brain from AAV-treated GM1 mouse, or control non-injected GM1 mice was fixed for 10 min in 0.25% glutaraldehyde in phosphate buffered saline (PBS) at room temperature followed by two washes in PBS.
Pancreas tissue from BALB/c mice was fixed in 2.5% (vol./vol)./vol
For whole-mount staining, mouse WAT from Tgm2+/+ mice was fixed in 10% neutral-buffered formalin.
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The lung tissues removed from the infected mice were fixed with 2.5% glutaraldehyde and post-fixed in 1% osmium tetroxide.
Segments of the distal colon from mice were fixed in water-free Methanol-Carnoy's fixative [60% dry methanol, 30% chloroform and 10% acetic acid].
Briefly, adult mice were fixed with paraformaldehyde; the muscles were removed, post-fixed, rinsed and mounted on slides.
For histological analysis, liver tissue specimens from CCl4-injuried mice were fixed in 4% PFA overnight, then dehydrated by graded ethanol at RT and embedded in paraffin.
Limbs of P1 mice were fixed with 4% PFA overnight.
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