Wells of a 96-well plate were pre-coated with 40 μl of Matrigel, into which freshly dissected ScW fragments from 10 12-week-old 10 12-week-old 10 12-week-olddded, and another 40 μl of Matrigel WTs added orer the ScW fragment.
Briefly, 4% paraformaldehyde (Sigma, USA) fixed tissue from transcardially PBS perfused mice were embedded in paraffin using an Excelsior tissue processor (Thermo, USA) and sectioned at 5 µm.
Brains from adult MeCP2−/y and wild-type mice were embedded in Tissue-Tek OCT compound (Sakura) and frozen in dry ice-cold isopentane.
Finally, islets from 12-weeks old RIP1-Tag2 mice were embedded in collagen to ascertain whether they harbored angiogenic properties ex vivo.
Tissues from moribund mice were embedded in paraffin and sections (10 μm) were H&E stained.
Pancreata from wild-type and Pten+/− mice were embedded in paraffin.
The aortic root and brachiocephalic artery of mice were embedded in optimal cutting temperature.
The formalin-fixed liver tissues samples from the treated mice were embedded in paraffin.
Pancreases from NOD mice were embedded in tissue-freezing medium (Jung) and stored at −20°C.
The decalcified ME bullae of mice were embedded in paraffin and sections were cut at 7 μm.
