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Immunohistochemistry. Mice were sacrificed and the proximal aorta of individual mice was dissected free of adipose and connective tissue, and immediately fixed in 4% paraformaldehyde.

Gene Expression Profiling of Dorsal Forebrain from Lhx6-Null Mutant Mice Dorsal forebrain from E15.5 embryos resulting from intercrossing Lhx6 +/− mice was dissected and stored in RNAlater (QIAGEN).

Briefly, whole striatum from WT, KO control and KO treated mice was dissected and frozen in liquid nitrogen.

The olfactory epithelium of OMP-GFP mice was dissected as described previously.

Primary visual cortex (area V1) from anesthetized mice was dissected and homogenized by sonication.

Cortical tissue from postnatal day 3 or 4 (P3 or P4) wild-type or PS2 KO mice was dissected and cut into small pieces (∼1 mm3).

Prostates from transgenic mice were dissected using a dissection microscope, fixed in 10% buffered formalin and embedded in paraffin.

Limb muscles from P10-P14 wild-type mice were dissected and subjected to a gentle collagenase (Sigma) and dispase (Gibco) digestion.

Spleens of asymptomatic mice were dissected 3 weeks after transplant in B6/SJL mice (CD45.1+), and cells were treated with ACK buffer as described above.

After the scans, the mice were dissected, and the abdomen was systematically and meticulously examined for tumors.

The euthanized mice were dissected, the organs were weighed, and the radiation in each organ was measured using a PerkinElmer gamma counter.