A week after surgery the mice were connected to flexible cables for recording sleep.
After a tracheostomy, the mice were connected to the flexiVent system (SCIREQ).
Genetic disruption of the MOV10L1 RNA helicase domain in mice is connected with a lack of piRNAs and male sterility.
One week after surgery, mice were connected to a 16-channel Medusa Preamplifier and filtered signals (300-5000 Hz) were acquired using a RZ5 Signal Processor (Tucker Davis Technologies).
After parabiosis, the circulations of the parabiotic mice were connected, and thus Aβ species in the blood of the parabiotic AD mice could be transported to the wild-type mice for clearance.
Two days following surgery, wild-type and GHS-R1A KO mice were connected to a microperfusion pump (U-864 Syringe Pump, AgnThós AB, Lidingö, Sweden) and perfused with Ringer solution at a rate of 1.5 µl/min.
Following anaesthesia (75 mg/Kg ip pentothal sodium), placement of tracheostomy and muscle paralysis (8 mg/Kg ip succinyl choline), mice were connected to a Flexivent rodent ventilator (Scireq, Montreal, Ontario, Canada).
Here mice were connected through a nose cone to breathe in anaesthetic gas, isofluorane (Forane, Abbott, Abbott Park, IL, USA) at 2 4% in O2, and breathe out excess anaesthetic gas to the same nose cone.
The mice were connected to the EEG Stellate Lamont Pro-36 programmable amplifier (Lamont Medical Instruments, Madison, WI) using a flexible cable with free access to all corners of the cage, including food, for 2 hr periods on 2 consecutive days (4 hr total), during the middle of the light cycle (typically between 9 AM and 4 PM).
Intubated mice were connected to a MiniVent rodent ventilator (Harvard Biosciences, Hollison, MA, USA) and mechanically ventilated with a tidal volume of 10 mL/kg, a respiratory rate of 150 breaths per minute, a fraction of inspired oxygen (FiO2) of 0.21, and no end-expiratory pressure.
