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Blood from CLP or sham-operated mice was collected in K2EDTA blood collection tubes and centrifuged at 4°C for 15 minutes at 2,000xg within 30 minutes of collection.
In addition, the whole blood of Balb/c nude mice was collected and centrifuged (1500 rpm, 3 min) to separate the red blood cells (RBCs).
(B and C) BM from the RBP-J cKO and control mice was collected 2 weeks after the UUO, and the CD11b+ monocytes were analyzed by using FACS for the expression of CCR2 (B).
Serum from non-pregnant vaccinated and non vaccinated mice was collected for the cytokine analysis.
Periovarial WAT from female HFD-fed mice was collected and analyzed by transmission electron microscopy (TEM) (Figure 7A).
Peripheral blood from mice was collected by cardiac puncture in a heparin-coated syringe, and red blood cells were lysed with Red blood Lysing buffer (Sigma-Aldrich, St . Louis MO, USA).
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Peripheral blood samples obtained from the tails of the mice were collected in heparin-coated quantitative blood collection tubes.
Male new born (NB) mice were collected within 24 hours after delivery and used for collection of the same tissues.
While most of the native mice collected over two years were found in no-cat areas, 79% of all the house mice were collected in areas colonized by the cats.
(D) Primary bone marrow cells from WT or CD177−/− mice were collected and cultured in medium.
The tumours and organs of mice were collected, and a histopathological analysis was carried out.
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