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The health status of infected mice was checked daily.
The genotype of the mice was checked using the following primers and PCR conditions: lacZ rose gene (forward: 5'-ATCCTCTGCATGGTCAGGTC-3', reverse: 5'-CGTGGCCTGATTCATTCC-3', 315 bp), endogenous gene (forward: 5'-CAAATGTTGCTTGTCTGGTG-3'; reverse: 5'-GTCAGTCGAGTGCACAGTTT-3', 210 bp).
As for the adverse effects of injection of cahtepsin L inhibitor, when body weight, health condition, and a histopahtological examination of all organs from CatL-inh-administerred mice was checked, there was no significant change.
The dissection of an equal number of bilateral areas from both wild type and knockout mice was checked by examination of the adhesive lid at the end of every laser capture microdissection session.
The viability status of the mice was checked daily.
The health status of tumor‐bearing mice was checked on a daily routine.
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The mice were checked daily including weekend.
Genotypes of mice were checked by allele specific PCR from tail genomic DNA [21].
Female mice were checked for vaginal plugs each morning, and litter sizes were recorded on delivery during two successive matings.
Mosquitoes that fed on mice were checked for the presence of a blood meal in their abdomen and sporozoites in their salivary glands.
After the injection, mice were checked daily for plasma glucose concentration via the tail vein with a blood glucometer (Roche diagnostic, Meylan, France).
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