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Each mice was boosted twice at a 2-week interval with equal amount of immunogen mixed in the same ratio with incomplete Freund's adjuvant (Sigma-Aldrich, St . Louis MO, USA).
One month later, during consecutive days (30, 31, 32 days), each group of mice was boosted intramuscularly (i.m).
Each group of mice was boosted on day 14 with identical formulations, and serum was collected over time for analysis of titers of anti-OVA IgG.
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The mice were boosted three times at one-month intervals.
Fourteen days after primary immunization, mice were boosted with their respective antigens.
CFA recipient mice were boosted with their respective antigens emulsified in IFA (Sigma-Aldrich).
At week 30, all mice were boosted i.m. with dose of 0.5 µg HA H5N1 non-adjuvanted inactivated virus vaccine.
At day 80 after priming mice were boosted and the IgG anti-NIP titer was determined at day 94.
On Day 30, all mice were boosted via injection of the same immunogens into their left, rear footpads (Fig. 3A).
Thereafter, the mice were boosted twice with 50 µg Ndk-Alum mixture after intervals of 14 days.
After 2 weeks, mice were boosted with the same amount of protein with incomplete Freund's adjuvant.
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