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The expressions of the proteins were corrected by beta-actin density, and the expression in tissues of normal mice was arbitrarily set at 1.0.
For the qPCR analysis: mRNA levels were standardized to cyclophilin; expression in the PPARα-null mice was arbitrarily set to 1. Significance was determined by a Bayesian t-test (array data) or unpaired student's t-test (qPCR data), * = p-value <0.05.
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The experimental laboratory mice were arbitrarily divided in six groups each containing six mice.
Then, mice were arbitrarily placed in control (n = 7) and in each BPs treated group (n = 7).
Then, mice were arbitrarily placed in control (n=10) and NaPaC-treated group (n=10).
Since the mice were arbitrarily labeled, we used the permutation of mouse identities that gave the smallest value of D JS between two groups.
When tumors reached a volume of 200 mm, the mice were arbitrarily assigned to different groups (n = 6 each) to receive intratumoural injections of 10 PFU Ad-NDRG2, Ad-LacZ, or PBS.
Based on our observations of mammary tumor development [ 21], MMT mice were arbitrarily stratified into three age groups for purpose of experiment, including: 20 to 35 days (hyperplastic lesion), 53 to 87 days (mammary intraepithelial neoplasms (MIN), a premalignant stage), and 98 to 170 days (malignant stage) and compared with control MT and MUC1.Tg mice.
When the tumours reached a volume of approximately 200 mm, the mice were arbitrarily assigned to different groups (n = 6 each) to receive intratumoural injections of 0.5, 1 or 2 × 10 plaque-forming units (PFU) of Ad-NDRG2, 2 × 10 Ad-LacZ or PBS.
Mice were arbitrarily placed in the groups of treatment.
For these mice, threshold was arbitrarily assigned to be 95 dB SPL.
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