Body composition of non-anesthetized mice was analyzed by Quantitative Magnetic Resonance imaging (QMRi) using an EchoMRI 3-in-1 composition analyzer (Echo Medical Systems, Houston TX).
The phenotype of the mutant mice was analyzed at P6 and tamoxifen injected Cre− littermates were used as controls.
Urine of castrated and castrated plus testosterone-treated male mice was analyzed using gas chromatography linked mass spectrometry (GC MS).
In a disseminated MOLM-13 xenograft model, tumor burden in the peripheral blood, bone marrow and spleens of control and BAY 2402234-treated mice was analyzed after 8 days of treatment.
Biodistribution of heparin-coated ADSCs upon intravenous injection in mice was analyzed by In-Vivo Imaging System (IVIS), and showed enhanced accumulation in the liver and spleen while reduced entrapment in the lung.
Pulmonary vascular remodeling of the mice was analyzed as we have previously described [7], [15] [18].
Total RNA from the eyes of Cacna1fwt and Cacna1fnob2 mice was analyzed by RT-PCR.
βgal enzymatic activity in the CNS of treated and control mice was analyzed both qualitatively and quantitatively (Fig. 2).
The histopathology of knock-in mice was analyzed by hematoxylin and eosin (H&E) staining, immunohistochemistry and electron microscopy.
Transgene expression in brain and spleen of these mice was analyzed by Western blotting using anti-PrP antibody POM1 [15], and mouse monoclonal anti-myc antibody 9E10.
Expression of PCNA by microglia isolated from sham- and TMEV-infected mice was analyzed at days 3, 5, and 7 post-infection.
