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Successful wt TP53 induction in Dox-treated mice was again confirmed by immunohistochemistry, as described above.
In the NSCLC model, the incidence and tumor types in the chimeric mice was again identical to the incidence and tumor types observed in the original strain.
However, MLKL deficiency in a newly generated knockout mice was again shown to be responsible for the protection in the CIP model.
Virus load at 30 days was comparable in spleens (Fig. 1K) and salivary glands (Fig. 1L) of WT and 4-1BB−/− mice, suggesting that impaired T-cell inflation in 4-1BB−/− mice was again not a consequence of reduced antigen abundance.
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A month or two after treatment stopped, all of the mice were again able to father as many pups as control mice, and the animals' testis size and sperm counts returned to normal levels between 1 and 3 months after treatment stopped.
Immature thymocytes similar to the K14CreERxRac1flox/flox tamoxifen treated mice were again demonstrated (Supplementary Figure S1M O).
AMPA microinjection was performed at CT14 and mice were again housed in DD until CT15 when mice were sacrificed.
On p.i. day 3, mice were again injected with 150 µg agonistic anti-mouse 4-1BB mAb or with 150 µg rat IgG2a isotype control Ab.
LC3-II levels in the cerebellum of chronically treated Npc1−/− mice were again decreased when compared to untreated controls and very similar to WT levels, analogous to results of the short term study.
At times t60 and t120, mice were again transported to the adjacent room, bled from additional tail incision for a third and fourth time to obtain blood samples for analysis of recovery from the stressor, and returned to the colony room.
In the first trial, one arm of the Y-maze was closed with a guillotine door and mice (n = 9/experimental group) were allowed to visit two arms of the Y-maze for 5 min. After a 30 min inter-trial interval (ITI), the mice were again placed in the start arm for the trial 2 and allowed free access to all three arms for 5 min. Start and closed arms were randomly assigned to each mouse.
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