Because transgenic reporter mice utilize the firefly luciferase, it is possible to monitor infections with lux + bacteria within the same animal, given that the spectra for the bacterial and firefly luciferase are different, and the firefly luciferase produces light only when the substrate luciferin is provided.
A single, full-length Abp gene pair in each of the dog and cat genomes would be consistent with a pattern of increasing importance of Abp genes in conspecific communication, proceeding from potentially no importance in primates to low importance in carnivores to relatively high importance in murid rodents, with the mouse utilizing the most number and diversity of Abp gene pairs.
We have also employed ASO technology to phenocopy SMA in transgenic mice, utilizing ASOs that exacerbate the SMN2 splicing defect and persistently promote pathogenesis.
If not otherwise stated all mice utilized for the study were male, between the ages of 4 and 6 months.
We have shown significant repression of hepatic ERα activity in mice utilizing Ad.shERα vectors.
The number and group allocation of mice utilized for the present studies are shown in Table 1.
Number, strain and treatment of mice utilized for the present studies, given as total number (number of mice with lung mechanics measurements).
37 The reasons for these disparities in transepithelial Isc are unclear but could be related to the source of mouse colony, the age of the mice utilized (12 13 weeks versus 8 weeks), the rodent diets used (Dyets, Inc. [Bethlehem, PA, USA] versus Special Diets Services [Essex, UK]), as well as the severity of the obesity and insulin resistance.
A large number of conditional mouse alleles utilizing the Cre- loxP system have been generated.
In these studies, we generated a mouse model utilizing the Cre- loxP system to ablate CENP-F protein expression in a cardiomyocyte-specific manner (termed CENP-F −/− hereafter).
