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Consistent with the mRNA data, we further confirmed the trend that protein expression of Hif1α was upregulated in the pancreas of pVHL deficient mice utilizing a Hif1α ELISA assay (Figure 6B).
PS and PCS models were developed in mice utilizing a communication box according to the method of Ogawa and Kuwabara [ 25].
TLR2−/− mice were derived onto the 129S6 background by iterative backcrossing of B6.TLR2−/− with 129S6.RAG2−/− mice utilizing a marker-assisted, "speed congenic" breeding method 62, 63.
In these experiments, Willingham et al., 2012 test the safety and efficacy of anti-CD47 antibody treatment in immune competent mice utilizing a syngeneic model of mammary tumor growth in FVB mice.
To identify genes that may be selectively associated with IC-IM, we compared gene expression patterns in gastric fundic tissues derived from wild type and W/W V mice utilizing a gene microarray method [ 19, 20].
To identify genes that may be selectively associated with IC-MY, we compared gene expression patterns in jejunal tissues derived from age-matched wild type and W/W V mice utilizing a differential gene display method.
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We have shown significant repression of hepatic ERα activity in mice utilizing Ad.shERα vectors.
To gain insights into EpCAM function in vivo, we generated EpCAM −/− mice utilizing an embryonic stem cell line with a tacstd1 allele that had been disrupted.
The transgenic expression studies of nNOS in mdx mice utilized a skeletal muscle specific promoter [41], [42], and provided the basis for an assertion that nNOS protects myofibers by directly working on myofibers.
Mice lacking RIP140 exhibit a decreased respiratory exchange ratio (RER) in comparison to WT controls, indicating that the null mice utilize a greater proportion of fat as an energy substrate in agreement with the upregulation of fatty-acid oxidation genes.
We established the Q129 DRPLA transgenic mice by utilizing a unique phenomenon, en masse expansion of CAG repeats.
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