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Phenotypic data collected from mice utilized in these experiments can be accessed and downloaded from http://cgd.jax.org/datasets/expression/10strain.shtml.shtml
The mice utilized in these studies were either SCID mice (C57BL/6J-Prkdcscid/SzJ, female, 4-week old, Jackson Laboratory) or MDX/SCID mice (bred in the institution's animal facility by crossing C57BL/10ScSn-Dmdmdx C57BL/10ScSn-Dmdmdxratory, and C57BL/6J-Prkdcscid/SzJ male, female, 4-week old, Jackson Laboratory).
Heterozygous mice were intercrossed to produce the male − /− (mutant) and + /+ (control) mice utilized in this study.
All mice utilized in this study were genotyped for the wild-type or mutated NNT as described [39].
Our own telemetric blood pressure recordings show instead that mice utilized in our study have a modestly but not significantly increased systolic and diastolic blood pressure.
The number of mice utilized in this study was sufficient to detect at least one QTL explaining 5% of the variance in exercise capacity with a power of 50% (Belknap, 2003).Increasing the number of mice would likely have allowed us to detect more QTL with smaller effects.
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It is important to note that the hepatic-specific albumin-Cre driven SIRT1 knockout mouse utilized in our study is a permanent knockout model.
In contrast, the Gfap- luc mouse utilized in the present study, which differs from our dual luciferase mouse in several respects, reports a much larger and sustained increase in promoter activity.
The original description of tolerance induction through mixed hematopoietic chimerism in mice utilized lethal whole body irradiation as the preparative regimen for achieving mixed chimerism.
Mice were divided into four groups (for each replicate 5 mice were utilized in per experimental group and 3 mice were used for the negative control group) and inoculated as follows: 1) PBS (negative control), 2) WNV alone, 3) mosquito feeding alone, and 4) WNV following the feeding of uninfected mosquitoes.
The transgenic expression studies of nNOS in mdx mice utilized a skeletal muscle specific promoter [41], [42], and provided the basis for an assertion that nNOS protects myofibers by directly working on myofibers.
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