Further studies using other mouse strains that are more prone to diabetes-induced NTDs should serve to clarify this issue.
If using other mouse strains or targeting a different organ, a dose response should be carried out to determine the optimal PEI-DNA ratio.
Because the differences in Gh mRNA in the hypothalamus did not replicate prior studies using other mouse models and tissue from mPOA or arcuate nucleus, we examined GH protein in the arcuate, a subdivision of the hypothalamus.
Unfortunately, between B6 and JF mice, no SNPs were detectable in coding Zdbf2-specific exons 1, 2 and 5 (data not shown); therefore, further analysis is required to determine whether coding Zdbf2 variant is expressed in a parent-of-origin-specific manner in placental tissues, e.g., using other mouse strains.
Consequently, we were able to detect significant changes in both DA and serotonin metabolism at very early stages of HD pathology in R6/2 mice that were not displayed using other sacrificial methods.
Another distinct advantage is the larger size of the sheep relative to the rats and mice used for other TRALI models.
Largely similar results were obtained with both approaches, although p38 dependency using the inhibitor appeared greater than in the p38α-knockout MEFs, possibly because these cells can adapt during mouse development to use other pathways, or because some genes are dependent on p38β.
The total doses of naloxone, 10mg, in these experiments is comparable to previous studies on rats using chronic intrathecal and acute subcutaneous administration and other studies on mice using chronic subcutaneous administration.
Although Hamilton et al. [ 19] recently indicated that BALB/c inbred strain mice are susceptible to T. canis infection and it seems that larvae remain in brains of BALB/c mice for a longer time compared to other strains of mice, using ICR mice as an animal model is still adequate to study CT.
