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Murine glomeruli and tubular preparations were isolated from mice using magnetic separation as previously described [23].
In adoptive transfer experiments, splenic CD4+CD25+ Treg cells were isolated from wild-type mice using magnetic bead separation (CD4+CD25+ regulatory T cell kit; Stemcell Technologies).
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Indeed, there is evidence that wood mice use magnetic orientation [ 9, 10] Radio-tracking studies in cereal fields makes it clear that wood mice do not always confine their movements to a restricted network of paths [ 5, 11], although during exploratory searches in the vicinity of a nest, wood mice have been observed to travel repeatedly on trails [ 12].
O'Leary-Moore and colleagues (2011) recently reviewed the different brain changes following a single day of alcohol exposure during early fetal development in the mouse using magnetic resonance imaging (MRI).
Following stimulation, BMDCs were plated (1 2 dilution) with purified CFSE-labeled CD8+ or CD4+ T cells isolated from spleens of OT-I or OT-II mice, respectively, using magnetic beads (STEMCELL Technologies), according to manufacturer's instructions.
Briefly, freshly isolated cell suspensions were prepared from male rat or mouse pituitaries using magnetic activated cell sorting (MACS; Miltenyi) or a fluorescence-activated cell sorter (FACS; FACSAria, Becton-Dickinson).
HIV protein identification in serum was performed by immunoprecipitation with anti-HIV P24 monoclonal mouse antibodies using magnetic beads coupled with protein A (Dynabeads Technology, Invitrogen, Carlsbad, CA, USA).
Splenic B cells were isolated from naïve mice using CD19+ magnetic beads (Miltenyi Biotec) and cultured with 10% plasma taken from mice infected 16 hours earlier with LDV.
DCs and CD8+ T cells were obtained from spleen cells of CBF1 mice using the magnetic cell sorter system MACS according to the manufacturer's protocol using anti-CD11c and anti-CD8 antibody-coated microbeads (Miltenyi Biotec), respectively.
Splenic B cells were isolated from naive female C57BL/6 mice using standard magnetic sorting techniques.
Lymphocyte subsets, namely CD4+ T-cells, CD8+ T-cells, CD3+ T-cells, CD19+ B-cells, and CD138/B220/CD19+ plasma cells were purified from spleens of CB-17 mice using immuno magnetic selection (Miltenyi Biotec) as per the manufacturer's directions.
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