After 3 weeks, the researchers induced seizures in the mice using either a drug injection or electrical stimulation of the brain.
Excess inflammatory cell infiltration and neointimal hyperplasia (accelerated plaque growth) were detected in aortic allograft transplants at 4 weeks follow up in control, saline treated, wild type (WT) mice, using either C57Bl/6 donor to Balb/c recipient mice (Fig. 1A,E) or Balb/c donor to C57Bl/6 recipient mice (Fig. 1 C, E, n = 19).
Supporting the relevance of this approach, it was shown that inhibition of Hif1 in mice using either pharmacological inhibition or conditional gene deletion significantly reduces airway inflammation in models of asthma [ 24, 25].
Menin expression was assessed in the different prostatic lobes from Men1 wild-type mice using either IHC for menin or double IF with antibodies against menin and p63, a basal epithelial cell marker.
Protein and RNA were then isolated from IECs of STAT5IEC WT and STAT5IEC KO mice using either EDTA-based or LCM to determine pMLC levels and TJ proteins (TJPs) expression.
The power of ENU (and ethyl methanesulfonate) as a tool for the high-throughput functional annotation of gene sequences has been applied to two primary mutagenesis strategies in mice using either whole mice or mouse embryonic stem (ES) cells: the phenotype-driven mutagenesis screen [ 5- 22] and, more recently, the gene-driven mutagenesis screen [ 22- 30].
Pre-operative X-irradiation of s.c. implanted first-generation mammary tumours in C3H mice, using either 500 rad or two fractions of 350 rad, produced no improvement in the success of surgery in causing local control or in reduction of distant metastases.
As expected, no differences in cone density were found for the wild-type (wt) mouse using either methods.
We have demonstrated that human sCD59 delivered via a gene therapy approach can attenuate CNV in mice using either an adenovirus vector injected subretinally or an AAV vector injected intravitreally.
Overall, there was a statistically significant decrease in the incidence of arthritis in the H4R-deficient mice using either a Fisher's exact test (p<0.007) or a log-rank survival method (p<0.002).
