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This corresponds roughly to the areas of antisense intergenic transcription seen in transgenic mice (unpublished observations).
Although this often culminates in spontaneous ulcerative dermatisis in older mice (unpublished observations), none of the mice used in the current study displayed this condition.
Furthermore, we have observed enhanced survival and growth potential of HSC from Sirt1 deficient mice during culture in cytokine deprived media, and negligible differences in numbers of HSC between young Sirt1−/− and Sirt1+/− mice (unpublished observations).
It could be associated with RANTES-mediated activation of the immune system, such as the enlargement of the lymph nodes and thymus and infiltration of organs with CD45 cells in both wild type and S100A4 knockout mice (unpublished observations).
We treated a small group of mice with B Lymphocyte Stimulatory Factor (BLyS), a factor known to promote human B cell survival [34], [35] and B cell engraftment in NOD-scid IL2rγnull mice (unpublished observations).
It is likely that these differences result from the considerably lower (5 10 fold) levels of BAFF in NZB as compared to BAFF-Tg mice (unpublished observations) together with the presence of additional signalling abnormalities in NZB dTg lymphocytes.
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This superiority was independent of the ECTV infection as seen in vaccination experiments with naïve C57BL/6 mice (unpublished observation).
However, our preliminary data indicated that the reconstitution of 10×106 CD3+ T cells derived from FoxP3 knock-out mice (no FoxP3+ Treg-cells) resulted in a decrease in size of CRC in CCR6−/− mice compared with CD3+ T cells derived from wild type mice (unpublished observation), hints to promoting effect of CCR6+ Treg-cells in the development of CRC in mice.
Male TG mice were maintained for over a year without obvious differences in survival compared to WT mice (unpublished observation).
Since Jurkat cells itself do not expand and do not form tumours in mice (unpublished observation), we assessed the effect of Jurkat-TRAIL cells in an in vivo tumour model.
Since the number of circulating leukocytes is influencing their adhesion to the endothelium, we determined their numbers and found that they were similar in ApoE−/−FNfl/fl and ApoE−/−FNMxCre mice (unpublished observation).
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