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However, we were unable to elicit a measurable response from TKO mice under light adapted conditions (Fig. 1C), indicating that other aspects of its photoresponse were atypical of cones.
Briefly, mice under light isoflurane anesthesia were prepared for studies by insertion of polyethylene catheters into a femoral artery and vein.
We recorded spiking activity of neurons as well as local field potentials (LFPs) in A1 in head-fixed mice under light isoflurane anesthesia.
One must be cautious in translating the data from our experiments as a strict description of neuronal activity in awake animals, as our results were based on recordings from mice under light isoflurane anesthesia.
Echocardiographic measurements were performerd in young and old mice under light anesthesia (isofluorane 0.5 1%) with a Vivid-Q portable ultrasound system using a ILS 12 MHz transducer (GE Healthcare, Wauwatosa, WI, USA).
We added discussion of the effect of anesthetics on auditory responses in the Discussion: "One must be cautious in translating the data from our experiments as a strict description of neuronal activity in awake animals, as our results were based on recordings from mice under light isoflurane anesthesia.
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Overall, these results suggest that CR during the daytime affects the temporal organization of the SCN clockwork and circadian outputs in mice under light-dark cycle.
Loss of light-dependent phosphorylation of Grb14 occurred in the Rpe65-/ mice, but Grb14 phosphorylation occurred in the wild-type mice under light-adapted conditions.
Unlike RF, the effect of IF on circadian rhythms in αMUPA mice was similar to that of WT mice under light-dark or disruptive light conditions, and it resembled also that on C57BL mice.
The positively stained apoptotic cells were counted randomly in five microscopic fields at least of each of the three slides per aorta (mouse) under light microscopy.
For Ki-67 staining, the positively stained nuclei were counted randomly in five microscopic fields at least of each of the three slides per aorta (mouse) under light microscopy.
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