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In contrast to SREBP1c, levels of SCAP are comparable in Crtc2+/+ and Crtc2−/− mice under fasted conditions (Fig. 1A).
Consistent with this observation, plasma glucose concentrations were significantly reduced in both male and female GHRH-KO mice under fasted conditions.
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Murine fundus without epithelial tissues derived from wild type and W/W V mice maintained under fasted conditions were used to generate poly (A)+ mRNA for microarray analysis using the mouse GEM 1 microarray.
Circulating concentrations of FGF21 are ∼5 10-fold higher in the FGF21-Tg mice than under fasted conditions.
To confirm the differential expression profiles, we further examined the expression levels in murine fundic mRNA derived from wild types and W/W V mice maintained under fasted conditions as templates, using semi-quantitative RT-PCR analysis (Representative data were shown in Fig. 1C,1D. All primer sets used for the confirmation were listed in Table 1).
To test whether other enzymes of the β-oxidation might compensate for the deleted hadh, the expression levels of Acadl (long-chain acyl-CoA dehydrogenase), Acadm (medium-chain acyl-CoA dehydrogenase), and Hadha (long-chain hydroxyacyl-CoA dehydrogenase) were quantified in liver of control and hadh−/− mice under fed (HFD) and fasted conditions.
However, blood glucose levels under both randomly fed and fasted conditions were lower, whereas plasma non-esterified (free) fatty acid levels were slightly higher under fasted conditions in the AS160-knockout mice compared with the wild-type littermates (Table 1).
In fasting/refeeding experiments mice were either fasted overnight or fasted followed by 5 h of free access to food.
Yet, the transgene not only does not engender such an effect under fasted conditions, it worsens glucose incorporation in GHR-KO mice (Fig. 3B).
These differences may be attributed to the fact that the compensatory rise in insulin was blunted in HF-fed AOiGHD mice, resulting in a significant difference in insulin levels between AOiGHD and controls, under fasted and fed conditions (Fig. 4B).
The plasma HDL-to-total-cholesterol ratio was higher, suggesting increased reverse cholesterol transport or cholesterol clearance in scallop-fed mice in both fasted and non-fasted states.
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