Sentence examples for mice under deep from inspiring English sources

Six weeks after the injections, we transcardially perfused the mice under deep anesthesia, with phosphate-buffered saline (PBS) followed by 10% formalin (Fisher Scientific).

Hemibrain or hippocampal samples were taken from the mice under deep isoflurane anesthesia and were snap-frozen for biochemical assays.

Adult mice under deep anesthesia were transcardially perfused with cold PBS followed by cold fixation buffer containing 4% paraformaldehyde in PBS.

Cochleae from the temporal bones of 3-day-old Occ +/+ or Occ −/− mice under deep anesthesia with ether were dissected in PBS without calcium.

For in vivo studies, the diffusion chambers were implanted in the abdomen or in the flank of mice under deep anesthesia (2.5 mg Ketamine + 0.5 mg Xilazine in 100 μl saline solution/mouse).

At Emory University, 3- to 4-month-old Scn8a med /+ and Scn8a med-jo /+ male mice under deep isoflurane anesthesia were surgically implanted subdurally with four sterile 0-80x3/32 screlectrodesdes for EEG analysis.

However, lethality was only observed in mice infected under deep anesthetic, since mice infected under lighter anesthesia conditions showed less dramatic weight loss (∼7%) and no mortality (data not shown).

Lumbar DRGs were isolated from mice decapitated under deep plane anaesthesia with carbon dioxide, and digested with 0.2% collagenase for 30 min and 0.05% trypsin for 15 min at 37 °C followed by gentle trituration with a fire-polished Pasteur pipette.

Spleens were dissected from mice (ICR) under deep diethyl ether anesthesia and were homogenized in an ice-cold Teflon-glass potter homogenizer containing 9 vol (w/v%) homogenizing buffer (0.25 M sucrose, 5 mM Tris-HCl, pH 7.4) and protease inhibitor cocktail (1 mM phenylmethylsulfonyl fluoride, 10 µM pepstatin A, 10 µM leupeptin) as previously described [39].

The other half of these mice and all hTgn mice were sacrificed under deep anesthesia, then the brain, spinal cord, and spleen were removed.

To prepare parotid gland-defective mice, the parotid glands of 7-week-old C.B-17/lcr-scid/scidJcl female mice were extracted under deep anaesthesia.