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The ability to study genetically diverse, wild-derived mice under controlled laboratory conditions has opened up several constructive research programs centered on understanding the phenotypic consequences of natural genetic variation.
Intratracheal instillations have been performed in mice under controlled general anaesthesia to avoid pain and discomfort.
In response to oxidative stress, mC has been demonstrated to be dynamically modulated in yeast [ 9, 10] and mC plays an important role in regulating tRNA stability and translation in mice under controlled conditions [ 11].
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The mice were housed under controlled environmental conditions with free access to water and food.
The mice were bred under controlled environmental conditions with free access, illumination was provided between 0800 and 2000 hours.
All procedures involving experimental mice were performed under controlled laboratory conditions in strict accordance with the Lithuanian and European legislation.
Wild-type male C57B16 mice were housed under controlled, specific pathogen-free conditions at the Shared Aging Rodent Facility at Madison VA Geriatric, Research, Education, and Clinical Center.
6-week-old C57BL/6 mice were acclimated under controlled specific pathogen-free (SPF) conditions for 1 week prior to the start of the experiment.
The mice were kept under controlled laboratory conditions with temperature at 25 ± 2°C, relative humidity of 60% ± 5%,and a light-dark cycle of 12 h each.
Indeed, Kirchhof et al. (2004) showed that KN-93 prolonged epicardial APD in mice under control, normokalaemic conditions.
The mechanisms described above, resulting in elevated body temperature of hadh−/− mice under control conditions (20 C), are obviously insufficient to facilitate adaptive thermogenesis.
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