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We then did a follow-up analysis of those mice two months after the injections.
Female Tg 189N3 mice, two months of age, showed a 1.4-fold increase in gene expression of Dyrk1a compared to non-transgenic mice (Fig. 1B).
Male Tg 152F7 mice, two months of age, showed a two-fold increase in gene expression of DYRK1A in the liver compared to non-transgenic mice (Fig. 1A).
Male Swiss mice (two months old) from the Animal House of São Paulo State University, Botucatu, SP, Brazil, were housed in groups of 3 5 animals and were provided with food and water ad libitum.
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For studies of immunocompromised mice, two month-old ICR-SCID male mice were obtained from Taconic and maintained and aged in a barrier facility.
Importantly, DA levels are reduced in Pink1−/− mice six months and older, which is associated with increased DA turnover.
Male Ts65Dn mice, six months of age, showed a 1.6-fold increase in gene expression of Dyrk1a compared to non-transgenic mice (Fig. 1D).
These results are in line with the previously published evaluation of hind paw analgesia in older Ins2Akita/+ mice, six months after the onset of diabetes [37].
To determine the contribution of BMDCs to fat tissue vessels, we performed lectin perfusion staining in WT/Actb-GFP-BMT mice four months post-BMT, and analyzed their abdominal fat tissue sections by confocal microscopy.
Male wild type C57Bl/6J mice, three months old, received stereotactic injections (AP −1.5 mm, lateral 1 mm, depth 2 mm) of 50 µmols of Tat1-72 or control vehicle in 2 µl, followed by sacrifice and intracardiac perfusion with paraformaldehyde 4 weeks later.
Wild type C57BL6/J male mice three months old at the beginning of the experiment were used.
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