Kinematic analysis of walking V1 IN-ablated mice revealed two factors that contribute to rear paw overshoot: (1) an increase in the speed and degree of ankle flexion movements during swing and (2) an increase in the angular rotation of the pelvis during stepping.
Studies in mice indicate that two factors interact to determine the number of meals and meal size.
Generation of iPS cells using retroviral vectors from short- or long-term cultured human and mouse amniocytes using four factors, or two factors in mouse, occurs in 5 7 days with 0.5% efficiency.
By day 5 post-infection (four factors, with mouse or human amniocytes) or day 7 post-infection (two factors with mouse amniocytes) in culture, colonies resembling ES cell colonies began to appear (Fig. 2A, E, I, Table 1).
In this section we describe another scenario with controlled experimental design where biological units (mice) are cross-classified according to two factors: Genotype (wild-type (WT) versus PPAR α deficient mice (PPAR)) and Diet (5 diets with different fatty acid (FA) compositions).
Lastly, our studies in the mouse system suggest that just two factors (Klf4 and c-Myc) are sufficient to initiate pluripotency, and the 2-factor mouse AdiPS cells were able to differentiate into all three germ layers.
Biological units (mice) are cross-classified according to two factors: genotype, which can be wild-type (WT) or PPARα-deficient mice (PPAR); and diet, with 5 classes of diet in accordance with the FA composition.
As Noggin and Sonic hedgehog (Shh) were recently shown to be required for early chick ventral skin formation, and able to trigger skin and feather formation from chick amnion, we associated cells engineered to produce those two factors with mouse amnion.
We theorize that the enhanced virulence of Stx2+ O157 H7 in mice fed an HFD results from two factors.
Given that defects in conduction are associated with mutations in NKX2-5 in both human and mouse models, our finding that the two factors share a common set of gene targets might account for these results.
