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Decreased tumor size following metformin treatment was due to the reduced proliferation status of tumor cells as shown by Ki-67 staining on mice tumor sections.
To search for more evidence of tumor cell apoptosis and necroptosis, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay) assay and ultrastructural study were also performed on mice tumor sections, respectively.
To investigate tumor vessel density in WT and EC JAM-C-KO mice, tumor sections from both strains were analyzed for VE-cadherin expression by immunofluorescent staining and confocal microscopy (Fig. 4 A C ) or were measured for the EC protein endomucin by Western blot (Fig. 4 D, E).
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More Ki-67 positive cells were detected in AT2-KO mouse tumor sections than in wild type mouse tumor sections.
To evaluate the killing mechanism of transfected cells treated with GCV, TUNEL assays were performed on mouse tumor sections.
To determine whether the molecular events observed in cell lines were also found in mice, we performed immunohistological staining and TUNEL assay of mouse tumor sections.
Consistently, melanoma blood vessels in DKK1 Tg mouse tumor sections stained less for beta-catenin (37 % reduction) compared with that in control mice (Fig. S1a, b).
Mouse tumor sections used for SEM as well as AFM studies were obtained by establishing tumors in nude mice treated with either control B16F1 mouse melanoma cells or with SMAR1-P44 peptide [ 42].
To verify the existence of a VEGF autocrine loop in SS, mouse tumor sections were examined histologically; expression of both VEGF-A and VEGFR2 was detected in the tumor (Fig. 3c).
We have studied the correlation of SMAR1 expression with cell surface smoothness in cell lines as well as in different grades of human breast cancer and mouse tumor sections.
GA carried out the sample preparation for and statistical analysis of the global gene expression profiling study, validation of data, immunostainings of the mouse tumor sections and statistical analysis, human squamous cell carcinoma cell lines characterization, migration and proliferation assay analysis, siRNA knock down, drafted and corrected the manuscript.
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