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In MCAT transgenic mice, transgene expression is directed by the ubiquitous CAG promoter [20].
In APP23 mice, transgene expression is sevenfold higher than endogenous APP. APP23 mice develop an AD-like pathology (ß-amyloid depositions) at 6 months of age.
In 3-month-old mice transgene expression in the growth plate could no longer be detected, but it still could be in articular cartilage.
In fact, upon HD-Ad injection through the tail vein of mice, transgene expression in livers has been shown to have life-long persistence [ 22].
In male mice, transgene expression was observed only in a few cells of the prostate, but not in the MG or any other tissues/organs (data not shown) and, therefore, no further analysis was pursued with MMTV-cortactin transgenic male mice.
In other work in our laboratory, we recently identified Mig13a/Lrp12 and showed that in Tg(Mig13a-EGFP) mice transgene expression is restricted to only those subplate neurons projecting to the anterior commissure, and is not expressed in those neurons projecting to the thalamus (S. Schneider and M.E. Hatten, manuscript in submission).
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MMTV LTR-TGF β transgenic mice have transgene expression and biological changes that are exclusive to the breast in female mice [ 18, 19] and prostate in male mice [ 20].
The resulting offspring were further characterized for transgene integration by PCR analysis and after crossing to C57Bl/6J wildtype mice for transgene expression by RT-PCR.
The resulting offspring were further characterized for transgene integration by PCR analysis, and after crossing to C57BL/6 J wildtype mice, for transgene expression by RT-PCR.
As all patient-derived glioblastoma stem cell cultures that efficiently grew in our hands were p53 mutant (M Vucikevic and ARA, personal communication), and as NS from DT+dox mice extinguished transgene expression in vivo after transplantation (not shown), we used human U87 glioblastoma (p53 wt) cells.
For the analysis of F1 generation mouse lines, transgene expression was determined by western blots in comparison with recombinant human SNCA (50 ng).
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