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AID transgenic mice were crossed with hTau mice to yield mice expressing AID transgene, human Tau transgene and one allele copy of mouse Tau.
This subclone was injected once more into the mammary fad pad of nude mice to yield a second cell line D3H2LN, harvested from a lymph node metastasis [15].
After lethal irradiation, Prp0/0, Tga20 or C57/BL6J congenic mice were reconstituted with BM from each of the other mice to yield nine different types of chimeric animals.
The offspring was crossed back with homozygous 3xTg-AD mice to yield quadruple transgenic animals homozygous for the knock-in mutation and the AD transgenes and heterozygous for YFP-H.
ACKR2-deficient mice were crossed with CD11cYFP mice to yield ACKR2-deficient/CD11cYFP mice.
These were then crossed with Hr6b+/- males derived from backcrosses of Hr6b+/- mice with FVB mice, to yield male Hr6b-/ mice.
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Wow! – another word you won't find used by Jane Eyre – a million books, all waiting, at the click of a mouse, to yield whatever you want them to yield.
Germ-line transmitters of the mutated TnfrI allele were crossed with C57BL/6 mice and the resulting heterozygous mice interbred to yield homozygous mutant offspring.
Hr6b+/+ mice, derived from backcrosses of Hr6b+/- mice with FVB mice were intercrossed to yield male Hr6b+/+ mice.
F1 mice were intercrossed to yield mice homozygous for the Anaef mutation and carrying a mix of C57BL/6 and background CBA/J single nucleotide polymorphisms (SNPs).
Based on our preliminary dose-escalating experiments, we identified that TMZ (5 mg/kg) and 2 Gy/kg as minimally efficacious doses at multiple doses instead of one weekly dose and the schedule combination was chosen for our GBM mouse model to yield enhanced survival in combination with MMP14 attenuation.
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