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The U.S. Commerce Department destroyed more than $170,000 worth of desktops, printers, TVs, cameras and computer mice to remove from its network an infection that had been vastly overstated, according to a newly released audit.
These homozygous mice were crossed with EIIa-Cre[65] mice to remove DNA fragments between two loxp sites.
Mosaic, early embryonic, ubiquitous Cre mice (MeuCre40) [22] were crossed with Sp2wt/lzn mice to remove the LacZ-Neo selection cassette from the Sp2 tri-loxP-allele.
However, we were able to breed the female BcorNeo/+ mice to FLPe recombinase expressing mice to remove Neo, and got viable BcorFl/+ and BcorFl/Y progeny (Figure 2A).
F1 progeny mice carrying both the FLPo transgene and the Lox allele were backcrossed to wild-type mice to remove the FLPo transgene.
Mice that were shown to be heterozygous for the targeted allele were mated to Flip recombinase-expressing transgenic mice to remove the neo cassette.
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The entire social test box is washed under tap water and dried with absorbent paper between each test mouse to remove odours and the time spent in each lateral chamber during the habituation phase is recorded to check for place preference.
After obtaining germ line transmission in the Ascl1CreERT2-Frt-Neo-Frt mice, they were crossed with FLPe mice [10] to remove the neomycin cassette resulting in Ascl1CreERT2 mice.
Heterozygous offspring (PiT1neo/+ mice) were bred either to ACTB FLPe mice [24] to remove the neo cassette, leading to PiT1lox/+ animals, or to PGK-Cre mice [25] to produce PiT1Δ5/+ mice.
Founder mice were crossed with Cre-deleter mice [43] to remove the neo gene and backcrossed onto the C57Bl/6 line nine times.
These mice were then crossed to EIIa-cre transgenic mice [20] to remove the neo cassette that had been placed into the first intron.
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