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By generating K14-GFP-actin mice to monitor actin dynamics in cultured primary keratinocytes, we uncovered a role for the actin cytoskeleton in establishing cellular organization.
Using linear amplification-mediated PCR, we then analyzed vector integration in the bone marrow and CFC of the engrafted mice to monitor the clonal activity of the transduced SRC in vivo.
In an attempt to evaluate the level of attenuation of Kim53ΔnlpD, we used mice to monitor the colonization of internal organs by Kim53ΔnlpD and the wild type strain Kimberley53.
These tests were carried out in 3-month-old mice to monitor the phenotype.
Another possible explanation for this discrepancy may be the use of GFP-LC3 transgenic mice to monitor this process.
A method has been tested in laboratory mice to monitor for the presence of brevetoxins in blood after exposure.
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Water and food [2018 global Rodent Diet (Harlan)] were freely available for mice used to monitor activity in the home cage, but mice destined for the 5-CSRTT were subject to restricted diet (see below).
GFP-LC3 transgenic mice, engineered to monitor autophagosome formation, have been used in many studies of autophagy (Mizushima et al. 2003).
A thermocouple was inserted under the mouse skin to monitor temperature throughout treatment, which remained fairly constant throughout treatment.
To investigate whether IFNβ would also induce phagocytosis in the ex vivo cerebellar slice culture model we used the PLP-EGFP reporter mouse model to monitor the clearance of myelin debris in situ [ 20].
We derived two different strains for in vivo analysis: (i) a reporter mouse line to monitor miR-9 activity with single-cell resolution and (ii) a line for miR-9 loss-of-function studies.
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