The streptozotocin (STZ) was used in C57BL/6 mice to induce the type 1 diabetes mellitus (T1DM) for xenotransplantation models establishment.
A previous report crossed Tie2-Cre or VE-cadherin-Cre mice with Ctnnb1lox ex3)/lox(ex3) mice to induce EC-specific expression of β-catenin (Δex3) but these mice were embryonic lethal13.
The most potent chemical carcinogens require only a single injection in young mice to induce a high incidence of lymphomas, often thymic T-cell lymphoblastic lymphomas.
Therefore, we bred Tie2-Cre mice with PPARgamma(flox/flox) mice to induce EC loss of PPARgamma (Tie2 PPARgamma, and we assessed PAH by right ventricular systolic pressure (RVSP), RV hypertrophy (RVH), and muscularized distal PAs in room air (RA), after chronic hypoxia (CH), and after 4 wk of recovery in RA (Rec-RA).
Mouse breast cancer 4T1 cells were injected into the mammary fat pads of mice to induce primary breast tumor growth.
After one hour of administration of drug and extract, 0.6% glacial acetic acid (10 ml/kg) was given i.p. to all the mice to induce pain [13].
A single dose of freshly prepared STZ (180 mg/kg body wt). in cold 0.01 M citrate buffer (pH 4.5) was administered intraperitonially to overnight fasted mice to induce diabetes [15].
To study if Mecp2 silencing in intestinal epithelium was able to reproduce the phenotype observed in Mecp2-null mice, transgenic animals expressing Cre recombinase under the control of the Villin promoter (Vil-cre) were crossed with Mecp2 flox/y female mice to induce the deletion of loxP-flanked exon 3 and 4 of Mecp2.
ConA was injected into naïve C57BL/6 (WT) mice to induce hepatitis as described before.
We administered escalating doses of WBI to C57Bl/6J mice to induce RIGS.
Kainic acid was injected into the vitreous humor of C57BL/6 mice to induce reactive gliosis and death of RGCs.
