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We performed immunohistochemical analyses on primary tumors and metastases harvested from these mice to characterize palladin expression during disease progression.
We used four groups of mice to characterize the suppression of NK cells by Tregs: NK-undepleted, CD25-depleted (NK+CD25−); NK- and CD25-depleted (NK−CD25−); NK-depleted, CD25-undepleted (NK−CD25+) and NK-and CD25-undepleted (NK+CD25+).
In the present study, the authors use CTLs generated from Zap70(AS) mice to characterize the role of integrins versus the TCR in synapse formation and function.
In these experiments, we compared the effects of Cdk4 pathway blockade on the number and size of adenomas in the Apc −/+ Cdk +/+ with Apc −/+ Cdk4 −/− mice to characterize the role of the Cdk4 pathway in tumor development in vivo.
Most notably for the former, Gordon and coworkers have been experimentally manipulating germ-free, gnotobiotic and 'conventionalized' (provided with their normal microbiota) zebrafish and mice to characterize interactions with their microbial partners [ 6, 8].
He then determined that a conserved T-cell receptor (TCR) α chain (TRAV5D-4) is critical for this immune response (76– 78) and he engineered TCR transgenic mice to characterize its role (79).
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The data were analyzed individually to gain more insights on the neuroinflammatory response unique to the mouse, to characterize the spatiotemporal evolution of the lesion and to quantify the changes in lesion volume and length with time.
In this regard, it will be interesting to investigate IDO expression in different subsets of DCs from tolerized mice and to characterize their role in the induction and maintenance of immune tolerance.
We used in vitro cell migration and colonization assays and in vivo mouse models to characterize screening hits.
Our research group has performed microarray analysis of various normal mouse tissues to characterize transcriptional regulation and to identify potential biomarkers in response to IR exposure, specifically from i.v.-administered radionuclides used in cancer therapy [10 15].
Since HAdV-D22 was never associated with EKC, we performed whole genome sequencing, complemented with bioinformatics, including phylogenetic and in silico proteome analysis, as well as in vivo studies in a mouse model to characterize this unique recombinant virus.
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